The Dirty Diamonds tour launched in America in August 2005 after several European concerts, including a performance at the Montreux Jazz Festival in Switzerland on July 12. Cooper and his band, including Kiss drummer Eric Singer, were filmed for a DVD released as Alice Cooper: Live at Montreux 2005. One critic, in a review of the Montreux release, commented that Cooper was to be applauded for "still mining pretty much the same territory of teenage angst and rebellion" as he had done more than thirty years previously.
In December 2006, the original Alice Cooper band reunited to perform six classic Alice Cooper songs at Cooper's annual charity event in Phoenix, entitled "Christmas Pudding".
On July 1, 2007, Cooper performed a duet with Marilyn Manson at the B'Estival event in Bucharest, Romania. The performance represented a reconciliation between the two artists; Cooper had previously taken issue with Manson over his overtly anti-Christian on-stage antics and had sarcastically made reference to the originality of Manson's choosing a female name and dressing in women's clothing. Cooper and Manson have been the subject of an academic paper on the significance of adolescent antiheroes.
In January 2008, he was one of the guest singers on the new Avantasia album The Scarecrow, singing the 7th track "The Toy Master". In July 2008, after lengthy delays, Cooper released Along Came a Spider, his 25th studio album. It was Cooper's highest charting album since 1991's Hey Stoopid, reaching No. 53 in the US and No. 31 in the UK. The album, visiting similar territory explored in 1987's Raise Your Fist and Yell, deals with the nefarious antics of a deranged serial killer named "Spider" who is on a quest to use the limbs of his victims to create a human spider. The album generally received positive reviews from music critics, though Rolling Stone magazine opined that the music on the record sorely missed Bob Ezrin's production values.